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AC U PO Box 129, Tiverton, Devon EX16 0A.]
Telephone 01398 332 437 E-mail: acumenlab@-h0tmail.c0.uk
Acumen: 141846 Patient? Annette Hansen
Date of birth: 09/04/1962
Reported: 15/03/2014 DOCK)": Dr M MilZ Dr K Bieber
Blood metallothionein studies
Background. Metallothionein binds zinc, copper and toxic metals. in a number of metabolic processes it is the normai carrier-protein for zinc and copper. When it binds toxic metals such as cadmium the tendency is to form a very strong bond with the cadmium-MT complex that is then deposited in tissues — usually renal tissue. Even though, as radioactive-tracer studies have shown, the MT is exchanged over tinae, the ‘replacement‘ MT re-binds the cadmium and it remains in the kidney or other tissues if already ‘dumped’ there.
Test procedures: We first perform a screening test of functional MT function. A buffered preparation of the patient‘s albumin is ‘saturated’ with zinc and allowed to interact with the patients white cells that are imobilised on microscopic glass beads. After a washing procedure, the white cells that have been attracted to the beads are lysed, stained and inspected microscopically. The results are compared with those from normal controls.
If abnormal MT induction is suspected from the above and when we have available suitable anti-sera*, we proceed to measure the total serum MT level using an ELISA (enzyme—linked) analytical method.
*Please note that it is not always possible to obtain this anti-sera.
Results: The functional (screening) test: High
ELlSA assay results (serum): ' ' I Reference range “6' Result = 410 ug/I 84 to 360
Zinc = 16%, copper = 69%, cadmium = 6%, mercury = 5% and there are
traces ofnickel pailadium. Mb M 6 A: 0” 3L
cam Normaily, around 40% of the total metal on metailothionein is zinc. Copper accounts for most ofthe remaining 60%. When present, toxic metals can displace zinc or copper but poor zinc—availability and the presence of inflammatory changes also iead to a poor zinczcopper ratio. With the exception of mercury, metals present are measured using Metrohrn 797 VA Computrace instrumentation and software.
Dr John McLaren-Howard DSc FACN - Directors - Mrs Mirhane McLaren-Howard
Acumen Medical Ltd trading as Acumen Registemd in Engiand and Wales, no: 7082142 Registered Office: Key House, Woodward Road, Howden Industrial Estate, Werton, Devon, EX16 (UK)
Comments: As a % of the total metals on this metallothionein: